A systematic examination of the microbial world, with an emphasis on pathogens. Topics covered include morphology, physiology, and genetics as well as the metabolic and enzymatic reactions associated. Strategic techniques for the growth, isolation, and visualization of microbes are included as are microscopic and diagnostic methods. The causative agents and treatment strategies of various pathogens are emphasized. Viral composition, replication, and the associated disease states are also covered in detail. The laboratory component of this course is delivered using virtual labs with detailed instruction and demonstrations from an experienced microbiologist.
Prerequisites: General biology and general chemistry (recommended, but not required)
Module 1: Intro to microbiology, prokaryotes vs eukaryotes, cellular organelles and arrangements.
Module 2: Microbial metabolism and enzymatic reactions
Module 3: Microscopy and microbial staining techniques
Module 4: Microbial growth, universal, selective and differential growth media
Module 5: Personal protective equipment and pathogenic microbes
Module 6: Virus characterization, morphology and pathogenic causative agents
Lab 1: Notebook maintenance and general equipment
Lab 2: Microscopes
Lab 3: Gram Staining
Lab 4: Microbial cultivation and types of growth media
Lab 5: Antibiotic sensitivity testing
Lab 6: Enzymatic reactions
Lab 7: Secondary characterization assays
Lab 8: Biochemical assays
Lab 9: The unknown microbe
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